Quadram Institute Bioscience – Partner Institute

For inquiries, please contact Lisa Clark (lisa.clark@quadram.ac.uk) and Christian Roghi (christian.roghi@quadram.ac.uk).

Research Areas & Focuses

  1. Investigating the impact of phenotypic heterogeneity of clones within biofilms on community survival – Mark Webber
  2. Biofilm physico-chemical characterisation – Investigating the rheological properties of biofilms formed by motile and non-motile forms of C. jejuni – Pete Wilde
  3. Exploit the ability of commensal bacteria (Lactobacillus) to form biofilms as an alternative approach to combat pathogen adhesion on mucosal surfaces  (in humans and animals) – Nathalie Juge
  4. Exploit the ability of alginate oligomers to prevent and disrupt biofilms, with a focus on C. difficile infection in the gut – Nathalie Juge
  5. Exploit the ability of functional carbohydrates (human milk oligosaccharides) to act as receptor decoy for pathogens – Nathalie Juge
  6. Genomic epidemiology of antimicrobial resistance – Alison Mather
  7. Investigating bacterial attachment to material coupons – Arjan Narbad
  8. Biofilms on material coupons in the CDC bioreactor- investigating the influence of materials and the influence of leached chemicals from materials – Arjan Narbad
  9. Biofilm development on surfaces (applied microbiology; tap component surfaces) in vitro – Arjan Narbad
  10. Modelling the transfer of bacteria, bacterial (irreversible) attachment, survival and persistence in situ – Arjan Narbad
  11. Development of biofilm in a model water distribution system; investigating biofilm sloughing within the model system and impact on water quality – Arjan Narbad
  12. Investigating biofilm in hospital devices through destructive sampling, culture and microscopy techniques – Arjan Narbad
  13. Field investigations of the presence and/or effect of biofilm in medical devices (e.g. identification of contaminated components, sampling of the water and air (aerosols) contaminated by the biofilm presence) – Arjan Narbad
  14. Investigating bacterial presence by ATP swabbing methods – Arjan Narbad

Facilities

  • Measure biofilm viability in different conditions – Mark Webber
  • Developing models of biofilm adaptation and evolution to antimicrobial stress – Mark Webber
  • Measuring spatial and temporal expression of genes of interest within biofilms – Mark Webber
  • Determination of biofilm rheology and structure on surfaces – Pete Wilde
  • Microbial genomics and bioinformatics – Alison Mather
  • in vitro organ cultures based on intestinal biopsies / tissue (human in collaboration with NNUH/Biorepository or animals) to assay impact of strains/compound on biofilm formation ex vivo-mucus producing cell lines – Nathalie Juge
  • Germ-free mouse facility (to assess impact of specific strains on biofilm formation) – Nathalie Juge
  • Transgenic mice deficient in mucus/mucin glycosylation – Nathalie Juge
  • Analytical facility to assess mechanisms of interaction: bio-layer interferometry (Octet system), isothermal titration calorimetry, surface plasmon resonance (Biacore) – Nathalie Juge
  • Mucin purification -Nathalie Juge
  • Glycomics analyses (LC-MS, MS-MS) – Nathalie Juge
  • Static biofilm assays for basic biofilm forming ability comparisons of strains and mutants – as determined by absorbance – Arjan Narbad
  • Glass slide style biofilm investigations and antimicrobial testing using fluorescence microscopy (live/dead) percentage coverage – Arjan Narbad
  • SEM and fluorescence microscopy to visualise biofilms – Arjan Narbad
  • Flow cell systems for development of biofilms for microscopic analysis – Arjan Narbad
  • CDC Bioreactor (multiple uses e.g. biofilm development on coupons,  modelling under flow conditions (batch or continuous), exposure of biofilms to biocides/antimicrobials) – Arjan Narbad
  • We have the capacity to test strain biofilm forming ability which we plan to do on a large scale using the 96 well plate static biofilm method – Arjan Narbad

Researchers

Mark Webber
Pete Wilde
Alison Mather
Nathalie Juge 
Arjan Narbad 

Department Link

Quadram Institute Bioscience