CORE UNIVERSITY

University of Nottingham

nbic nottingham

Research Areas & Focuses

  1. Quorum sensing signalling and inhibition in biofilms
  2. Mechanisms of biofilm formation and dispersal
  3. Biofilm target identification, drug discovery and delivery.
  4. High throughput drug screening design and execution.
  5. Bespoke biofilm models: in vitro, ex-vivo and 3D printed.
  6. Infection biomarkers.
  7. Imaging: high throughput confocal, super-resolution, 3D metabolite and nanosensors.
  8. Machine learning and artificial intelligence for AMR big data analysis
  9. Polymicrobial biofilm and microbiome interactions with human and plant hosts.
  10. Mechanisms of biofilm surface interactions
  11. Antibiofilm polymer design for coatings
  12. Wastewater re-use: pollutants to products.
  13. Biological Engineering

University of Nottingham

For enquiries, please contact Miguel Cámara.

High Content Imaging

The Zeiss Cell Discoverer 7-LSM 900 is installed downstream of a robotic arm and multiplate incubator for efficient high throughput screening. It can deliver fluorescent images from widefield through to super-resolution with acquisition that can be target guided by machine learning. The 3D image acquisition is fast, automated and can be delivered from multiple points in realtime by virtue of the environmental control that covers both a temperature range and anaerobic conditions. Quantification is rapid and multiple sample formats are accommodated as well as cross-correlation with Electron Microscopy in the nmRC. It is housed in biological safety laboratory level 2 condidions for pathogen imaging and has a 20 minute sterilization cycle that can be deployed.

Robotic Platforms

The robotic suite is a custom-made system that consist of two interconnected robotic platforms located in the synthetic biology research centre (SBRC). The robotic system allows for the setup of customized protocols and workflows. It gives a wide range of options for gene assembly, microbial transformation (chemical and HT electroporation), colony plating and picking, transformant library screening, purification of nucleic acid, proteins and analytes, size section of DNA bands, high throughput electrophoresis and screening of compound libraries.

3D OrbiSIMS

A Time of Flight Secondary Ion Mass Spectrometer (ToF-SIMS) with hybrid OrbiTrap™ functionality, the ‘3D OrbiSIMS’ is the first of its kind in an academic setting. Based on the prototype at the National Physical Laboratory (NPL) the instrument combines the function of the two hybridised components to facilitate an unprecedented level of mass spectral molecular analysis for a range of materials (hard and soft matter, biological cells and tissues).

Managed Compound Library

The University of Nottingham has an established state-of-the-art fully automated and integrated Managed Chemical Compound Collection Facility.  The facility provides a screening resource for the identification of chemical starting points as drug leads against drug targets of interest.  The diversity of the compounds in the compound library (as evidenced by the low pairwise Tanimoto score), combined with their lead-like characteristics, makes the library an ideal source for primary screening of your drug target.