External Image Credits Front cover: ‘Making Waves’. This image captures Salmonella enterica adapting to life within a biofilm. Over successive generations, genetic changes ripple through the population, leading to more structured, resilient, and visually distinct biofilm patterns. This photograph highlights the dynamic beauty of bacterial evolution and the complexity that underlies even the simplest forms of life. Image by Eleftheria Trampari, Quadram Institute. Page 14: ‘‘A Golden Matrix’. A golden outreaching bacterial community with glistening tendrils. An overgrown bacterial culture grown on a Petri dish. Image by Chris Daniel, Birkbeck College - University of London. Page 15: ‘Branching Out – pathways to survival’. A biofilm macrocolony formed by a curli deficient Salmonella enterica serovar Typhimurium, stained using Ebbabiolight 680 optotracer. The image was taken on a Deltavision Elite microscope with widefield and phase contrast microscopy on a 10x objective, and altered using FIJI, with changes to the LUT, as well as brightness and contrast. Image by Ho Yu Liu and James Lazenby, Quadram Institute. Pages 18-26: Credit and description as noted below images. Page 27: ‘Release the spores!’ False coloured SEM micrograph of P. aeruginosa, S. aureus, and C. albicans polymicrobial biofilms grown on synthetic cystic fibrosis sputum media under anaerobic conditions. Image by Brogan Richards, University of Nottingham. Page 30: ‘Green Eyes (Microbial Orbes)’. When the photographer took a sample of their Trichormus variabilis (cyanobacteria) biofilm to perform an experiment, the mechanical action of their loop created those beautiful orbe-like spirals made out of long bacterial filaments. The photographer felt like those structures were “looking back at them” when they observed them under the microscope, which the photographer thought was a fun sort-of revenge from the microbial world. Image by Edwin Rodriguez, University of Edinburgh. Pages 51-54: Credit and description as noted below images. Page 55: ‘Biofilm Bonsai’. SEM image showing biofilm formation within an in vitro gut model, the MiGut, after 4 days. Biofilm was grown on a bioresin scaffold with a honeycomb pattern. The biofilm developing on the scaffold organises into a bonsai-like structure, shaped by gut microbial species. Image by Emily Dewhurst, Serena Berberolli, Anthony Buckley and William Davis Birch, University of Leeds. Resources NBIC PUBLICATIONS AND REPORTS NBIC EDUCATIONAL RESOURCES 59
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